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Addgene

We narrowed to 14,875 results for: pET

Showing: 8961 - 9000 of 14875 results
  1. pWPT-mEGFP

    Plasmid
    #190606
    Purpose
    Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a deletion was created in mCherry CDS impairing i…
    Promoter
    EIF1-short
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pLV-MNTS-1-p75

    Plasmid
    #182945
    Purpose
    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR
    Depositor
    Insert
    MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter and enhancer
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pLV-MNTS-1

    Plasmid
    #182944
    Purpose
    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR
    Depositor
    Insert
    MNTS-1 (NTF3 Human, Synthetic, Homo sapiens)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    This is the Human NT-3 with mutations on the matu…
    Promoter
    CMV promoter and enhancer
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pEPQD1CB0792

    Plasmid
    #177076
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Insert
    major latex protein-like (NmMLPL) from Nepeta mussinii
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pEPQD1CB0120

    Plasmid
    #177052
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Insert
    major latex protein-like (NmMLPL) from Nepeta mussinii
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pX330_ACTB-e1 sgRNA / hSpCas9

    Plasmid
    #172830
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid
    #172831
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pX330_Camk2a sgRNA / hSpCas9

    Plasmid
    #172839
    Purpose
    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid
    #172829
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pX330_ACTB-i3 sgRNA / hSpCas9

    Plasmid
    #172827
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. CMV-10xPP7-Qb model based

    Plasmid
    #158203
    Purpose
    Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.
    Depositor
    Insert
    10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 8961 - 9000 of 14875 results