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pENTR-2B-Nkx2-5 delta HD-G8

Plasmid

#98614

Purpose

Gateway entry vector NKX2-5 _HD

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 _HD (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Deletion of the homeodomain. Replaced by 8 glycin…

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1941

Plasmid

#82564

Purpose

High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple264

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_FKBP-HsKIF1C(886-1103)-mCh

Plasmid

#253322

Purpose

mammalian expression of the IDR domain (aa 886-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(886-1103) (KIF1C Human)

Tags

FKBP and mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_FKBP-mCh-FTH1

Plasmid

#253323

Purpose

mammalian expression of human FTH1 tagged with FKBP and mCherry. FTH1 self-assembles into a 24-merparticle. FKBP enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsFTH1 (FTH1 Human)

Tags

FKBP, mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_FKBP-HsKIF1C(349-1103)-mCh

Plasmid

#253321

Purpose

mammalian expression of the stalk+tail domains (aa 349-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

FKBP and mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_HsKIF1C(1-376)-NC-LZ-TagBFP-FRB

Plasmid

#253326

Purpose

mammalian expression of the motor domain of human KIF1C tagged with TagBFP and FRB. NC and LZ facilitate stable dimerization. FRB enables rapamycin-induced attachment to FKBP-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

TagBFP, FRB

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-KLF17-CRa1-MS2

Plasmid

#251117

Purpose

sgRNA1 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA1 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-KLF17-CRa2-MS2

Plasmid

#251118

Purpose

sgRNA2 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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FSW TMEM192-3xHA

Plasmid

#246729

Purpose

Neuron-specific expression of human TMEM192 with a tag of 3x HA on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

TMEM192 (transmembrane protein 192) (TMEM192 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

Synapsin

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1-Aoah

Plasmid

#233141

Purpose

Thy1.1 and Aoah overexpression

Depositor

Peng Jiang

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl hydrolase (Aoah Mouse)

Use

Lentiviral

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-trasHCknob6xHis

Plasmid

#237364

Purpose

pTwist CMV BetaGlobin encoding trastuzumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

trastuzumab heavy chain

Use

Affinity Reagent/ Antibody

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig9FcholeHA

Plasmid

#237367

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-9-Fc (SIGLEC9 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig9R120AFcholeHA

Plasmid

#237369

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc R120A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-9-Fc (R120A mutant) (SIGLEC9 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig10FcholeHA

Plasmid

#237370

Purpose

pTwist CMV BetaGlobin encoding Siglec-10-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-10-Fc (SIGLEC10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG coca-5HT3-IRES-GFP

Plasmid

#242193

Purpose

Expression vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-5HT3-IRES-GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

CAG

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUltra-EGFP-P2A-Tr-NRL

Plasmid

#239100

Purpose

pUltra-EGFP bi-cistronic lentiviral vector expressing EGFP and truncated NRL.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Use

Lentiviral

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1

Plasmid

#241276

Purpose

Yeast two hybrid vector expressing AD-HAB1

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

HAB1 (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α-Tr-NRL

Plasmid

#239096

Purpose

Expresses truncated NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Expression

Mammalian

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α-FL-NRL

Plasmid

#239095

Purpose

Expresses full length NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (NRL Human)

Expression

Mammalian

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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