We narrowed to 5,007 results for: lenti sgrna

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pGMC00007

Plasmid

#166725

Purpose

non targeting sgRNA

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

Non-targeting guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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SiC-V1

Plasmid

#133041

Purpose

SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Article

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.5’-LMNA-eGFP-KI

Plasmid

#170545

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for LMNA knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

BsmBI

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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UGPC

Plasmid

#138954

Purpose

Lentiviral vector for co-expressing Cre recombinase and sgRNA

Depositor

Nadya Dimitrova

Article

Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

U6 and PGK

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CROP-seq-opti

Plasmid

#106280

Purpose

A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-sgTAF12(human)#4.5

Plasmid

#105987

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12-HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-IRF4-Puro

Plasmid

#123323

Purpose

Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

Doxycycline inducible minimal CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-Cas9

Plasmid

#75346

Purpose

Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

GFP (via t2a)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-sgTel

Plasmid

#122659

Purpose

Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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