We narrowed to 7,031 results for: SIM

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pRExT2A-CT-BTy

Plasmid

#232209

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

Plasmid

#220223

Purpose

AAV-STARR-seq validation control vector with TFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Ad-HisFlag-USP19ER

Plasmid

#155244

Purpose

for generating adenoviruses expressing the ER isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Mutation

H118R- please see dep. comments

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (UUA)

Plasmid

#154785

Purpose

plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

UUA anticodon and G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00192

Plasmid

#34343

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10446 (Side Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Article

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_SC_4.26_STARR-seq

Plasmid

#220220

Purpose

AAV-STARR-seq screening vector with 4.26 promote

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

4.26

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS AF

Plasmid

#140023

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

Y271A, Y349F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-C-STAT1-mGFP-P2A-Puro

Plasmid

#199345

Purpose

express murine STAT1 protein fused with mGFP

Depositor

Massimo Mallardo

Article

Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

Insert

signal transducer and activator of transcription 1 (Stat1 Mouse)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA Q75L

Plasmid

#19719

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

GTPase deficient & constitutively active Gl…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pYPQ-Cas9-Act3.0

Plasmid

#178954

Purpose

It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

Plasmid

#60661

Purpose

Flp-dependent expression of Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX- Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Use

AAV

Promoter

EF1α

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

Plasmid

#246438

Purpose

All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

NmeCas9
Nme-sgRNA

Tags

HA tag, NLS, and SV40 NLS

Expression

Mammalian

Mutation

changed Aspartic acid 16 to Alanine, deleted amin…

Promoter

EF-1 alpha and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mDIS3-FLAG

Plasmid

#60046

Purpose

Mammalian expression of FLAG-tagged mouse DIS3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

DIS3 (Dis3 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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SIV_Rev

Plasmid

#236244

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Rev

Use

Lentiviral

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDIO-DSE-mCherry-PSE-MCS

Plasmid

#129669

Purpose

Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

Depositor

Beatriz Rico

Article

Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

Type

Empty backbone

Use

AAV

Tags

mCherry

Promoter

EF1a

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-E3

Plasmid

#242408

Purpose

Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-E3

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mx1201 PylRS

Plasmid

#154764

Purpose

plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-G1 PylRS

Plasmid

#154763

Purpose

plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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