We narrowed to 43,314 results for: cha;
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Plasmid#99114PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mRuby2DepositorInsertmRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-ER
Plasmid#214413PurposeER localization of CFAST fused to Halotag-ERDepositorInsertrat cb5 ERM
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-4GS-LaG-2
Plasmid#172764PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)DepositorInsertLaG-16-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB120
Plasmid#68574PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D rM3D mCherry SV40
Plasmid#92285PurposeAAV DREADD expressionDepositorInsertrM3D
UseAAVTagsmCherryExpressionMutationPromoterGfaABC1DAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTurquoise2
Plasmid#99113PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2DepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-LD)
Plasmid#214424PurposeDetection of ER-lipid droplet contact siteDepositorInsertFRB-V5
UseTagsCFAST-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform1
Plasmid#70173PurposeMammalian expression of hsOPA1 isoform 1DepositorInserthsOPA1 isoform 1 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mbYFPQS
Plasmid#80742Purposemembrane-targeted biosensor for monitoring changes in intracellular chlorideDepositorInsertYFPQS
UseTagspalmitoylation signal sequence of neuromodulinExpressionMammalianMutationH148Q (CAC to CAG) and V163S (GTG to AGC).PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS PKC theta
Plasmid#8426DepositorInsertMouse Protein Kinase C theta (Prkcq Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-R115A/R119A
Plasmid#80696PurposeExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-R115A/R119A
UseAAVTagsExpressionMammalianMutationArg115 and Arg119 both mutated to AlaPromoterCBAAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform7
Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorInserthsOPA1 isoform 7 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP
Plasmid#114369Purposeexpresses Flpo recombinase-dependent GtACR2DepositorInsertGtACR2-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-mScarlet
Plasmid#211330PurposeMammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)DepositorInsertProminin 1 (PROM1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Golgi-7
Plasmid#56590PurposeLocalization: Golgi, Excitation: 513, Emission: 527DepositorInsertGolgi (B4GALT1 Human)
UseTagsEYFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJEC726
Plasmid#190814PurposeCRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationS. pyogenes dCas9 codon optimized for Streptomyce…PromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Human, Synthetic)
UseTagsG-GECO1ExpressionMammalianMutationPromoterCMV Immdeiate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-MbPylRS
Plasmid#217361PurposeExpresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmidDepositorInsertM. barkeri pyrrolysyl-tRNA synthetase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1
Plasmid#171416PurposeFor the purifcation of human ULK1 complexDepositorInsertULK1 (ULK1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …ExpressionMutationPromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1A eIF4A3
Plasmid#83035Purposemammalian expression of eIF4A3DepositorInserteIF4A3 (EIF4A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGE011
Plasmid#71537PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-tdTomato
Plasmid#99116PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomatoDepositorInsertDIO-tdTomato
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mNeonGreen
Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMTH PKC alpha
Plasmid#8409DepositorInsertMouse Protein Kinase C alpha (Prkca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry
Plasmid#167902PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only