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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-STOP

Plasmid

#215840

Purpose

Expression of FLAG/HA tag

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK1-CXCR4

Plasmid

#215845

Purpose

Expression of CXCR4 target with perfect-match site

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

CXCR4 target with perfect-match site

Expression

Mammalian

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-p54

Plasmid

#215844

Purpose

Expression of FLAG/HA-p54

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human p54

Expression

Mammalian

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaD

Plasmid

#210448

Purpose

Express waaD under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaD

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaID

Plasmid

#210449

Purpose

Express waaI and waaD under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaI and waaD

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaJ

Plasmid

#210450

Purpose

Express waaJ under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaJ

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaK

Plasmid

#210451

Purpose

Express waaK under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaK

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaRK

Plasmid

#210452

Purpose

Express waaR and waaK under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaR and waaK

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaT

Plasmid

#210453

Purpose

Express waaT under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaT

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaW

Plasmid

#210454

Purpose

Express waaW under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaW

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaV

Plasmid

#210457

Purpose

Express waaV under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaV

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaVL

Plasmid

#210458

Purpose

Express waaV and waaL under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaV and waaL

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaX

Plasmid

#210459

Purpose

Express waaX under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaX

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Promoter

ADH1

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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