We narrowed to 29,111 results for: PLE;

1. pYO1101

   Plasmid

   #235708

   Purpose

   Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

   Inserts

   Beclin 1 (BECN1 Human)
   ATG14L (ATG14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234370

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

   Plasmid

   #234371

   Purpose

   T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis Ubi10 and MTAP1 (synthetic)

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-ATG13 (450-517)-ULK1 (836-1050)-His

   Plasmid

   #222825

   Purpose

   Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Use

   Tags

   6His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-ATG13 (363-517)-ULK1 (836-1050)-His

   Plasmid

   #222824

   Purpose

   Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Use

   Tags

   6His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-GST-ATG13 (363-517)-ULK1 (836-1050)

   Plasmid

   #222822

   Purpose

   Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Use

   Tags

   6His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

   Plasmid

   #222821

   Purpose

   Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Use

   Tags

   TwinStrep Flag and msfGFP-10His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR156-DuET

   Plasmid

   #213267

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR156 (GPR156 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPRC5C-DuET

    Plasmid

    #213297

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC5C (GPRC5C Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GPRC5A-DuET

    Plasmid

    #213295

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC5A (GPRC5A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GPR174-DuET

    Plasmid

    #213271

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR174 (GPR174 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GPR160-DuET

    Plasmid

    #213268

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR160 (GPR160 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GPBA-DuET

    Plasmid

    #213255

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPBA (GPBAR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBT9-FHIP

    Plasmid

    #198527

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP (FHIP1B Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent mutations in codons 353 and 651 as well as…

    Promoter

    ADH1

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid

    #194894

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA2
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid

    #194886

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1and2
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_TT 3xFLAG-MAVS-Ala271

    Plasmid

    #158631

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Q271A please see depositor comments below

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCUU

    Plasmid

    #159747

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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