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CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Plasmid

#132544

Purpose

Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

Plasmid

#71811

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

Plasmid

#191801

Purpose

Full length wt AtPCO4 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

Plasmid

#159487

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-human RNF146 RING-WWE (30-183)

Plasmid

#132611

Purpose

expresses human RNF146 RING-WWE (30-183) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 RING-WWE (30–183)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

Plasmid

#71812

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

Plasmid

#174715

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332, E151R, R154E

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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AIRAP 6: AIRAP(1-89).GST,V1.pcDNA3

Plasmid

#21796

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Mammalian

Mutation

AA 1-89

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH2 binding E2 variant L3A2-1

Plasmid

#248581

Purpose

N-term GST tag E2 variant selected for activated ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3A2-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH1 binding E2 variant L3A1-1

Plasmid

#248587

Purpose

N-term GST tag E2 variant selected for activated ARIH1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3A1-1

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag RNF14 binding E2 variant L3R14-1

Plasmid

#248589

Purpose

N-term GST tag E2 variant selected for RNF14

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3R14-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag HOIP binding E2 variant L3HP-1

Plasmid

#248590

Purpose

N-term GST tag E2 variant selected for HOIP

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3HP-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag HOIL1 binding E2 variant L3HL-1

Plasmid

#248855

Purpose

N-term GST tag E2 variant selected for HOIL1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3HL-1

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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EW216 PGK-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

Plasmid

#236131

Purpose

Plasmid encoding the ZNF250-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF250(1-4)-deImmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Monosiga brevicollis CBS-pair domain

Plasmid

#166435

Purpose

Express pGEX-6P-1 Monosiga brevicollis CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Monosiga brevicollis CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Naegleria fowleri CBS-pair domain

Plasmid

#166437

Purpose

Express pGEX-6P-1 Naegleria fowleri CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Naegleria fowleri CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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