We narrowed to 7,101 results for: tev

1. pEBB-BT

   Plasmid

   #36295

   Depositor

   Ezra Burstein

   Article

   Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

   Type

   Empty backbone

   Use

   Tags

   BT-tag (peptide sequence from P.freudenreichii tr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046901

   Plasmid

   #145725

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. p2CT-His-MBP-Rca_Cas13a_WT

   Plasmid

   #91870

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   RcaCas13a (RcaC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-HT_CtCHAD

   Plasmid

   #127785

   Purpose

   Expresses CtCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046764

   Plasmid

   #145673

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046940

   Plasmid

   #149261

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pMH-HT_SsCHAD

   Plasmid

   #127783

   Purpose

   Expresses SsCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2CT-His-MBP-Cam_Cas13a_WT

   Plasmid

   #91869

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   CamCas13a (CamC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2CT-His-MBP-Ere_Cas13a_WT

   Plasmid

   #91868

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   EreCas13a (EreC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046854

    Plasmid

    #145759

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046832

    Plasmid

    #145691

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. GRLF1

    Plasmid

    #36195

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains the Ras homolog domain only (amino acid …

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. p2CT-His-MBP-Lbu_C2c2_K1087A

    Plasmid

    #91864

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) K1087A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1087A

    Promoter

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MKK4

    Plasmid

    #29579

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4046907

    Plasmid

    #149262

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046953

    Plasmid

    #145764

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046891

    Plasmid

    #145749

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAcrF3

    Plasmid

    #89246

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    AcrF3
    

    Use

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046919

    Plasmid

    #145615

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pIC389

    Plasmid

    #37387

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    LGN (GPSM2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-364 and 524

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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