We narrowed to 13,996 results for: eng
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Plasmid#59332PurposeExpresses splitTVA-P2A-EGFPDepositorInsertsplitTVA950-P2A-EGFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianPromoterCAGAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4RFP-5premGRASP
Plasmid#52499PurposeExpresses TagRFP-T and presynaptic mGRASP componentDepositorInsertsTagRFP-T
presynaptic mGRASP
UseRabies virusExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
H9 F42A pAcGP67a
Plasmid#41810DepositorInsertH9 Super-2 (IL2 Human)
Tags8x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable SinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-Syn1-mCherry
Plasmid#222967PurposeMake lentivirus to express mCherry in syn1 expressing cellsDepositorInsertmCherry
UseLentiviralPromotersynapsin IAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v13
Plasmid#220940PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Magenta
Plasmid#160446PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Magenta
UseSynthetic BiologyMutationBioBrick sites removedAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyMutationBioBrick sites removedAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AksgRNA
Plasmid#121955PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAkCas12b single chimeric gRNA
ExpressionMammalianAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-mouse-ATP6-1397N.
Plasmid#212951PurposeExpresses TALED_Left-mouse-ATP6-1397N in mammalian cellsDepositorInsertTALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
ExpressionMammalianAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLS.491
Plasmid#184956PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
ExpressionBacterialMutationrpoB donor T1534CPromoterT7/lacAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1007 (pSL6)
Plasmid#191002PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1005 (pSL4)
Plasmid#191000PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1004 (pSL3)
Plasmid#190999PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
Tags6xHisExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3
Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW414hrpL-cIgfp
Plasmid#61439PurposepSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs34-CI-LVA-t-plam-30gfp-t
TagsLVAExpressionBacterialPromoterHrpLAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC39
Plasmid#62333PurposesgRNA + 1x PP7 with mCherry for mammalian cellsDepositorInsertssgRNA + 1x PP7
mCherry
UseLentiviralExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only