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pICH41531

Plasmid

#50321

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH77901

Plasmid

#50341

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-Nb-SpyCatcher

Plasmid

#243752

Purpose

For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Nb-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SNAP

Plasmid

#243741

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SNAP

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SpyTag

Plasmid

#243739

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-C1

Plasmid

#127395

Purpose

Expresses amcyan fluorescent gene

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA5_8xCTS-ECFP-pA

Plasmid

#200247

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3_8xCTS-ECFP-pA

Plasmid

#200245

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2_8xCTS-ECFP-pA

Plasmid

#200244

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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B7RR92

Plasmid

#163246

Purpose

Inducible expression of UniProt identifier B7RR92

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B7RR92

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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U4PUB6

Plasmid

#163213

Purpose

Inducible expression of UniProt identifier U4PUB6

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

U4PUB6

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A4YVE0

Plasmid

#163217

Purpose

Inducible expression of UniProt identifier A4YVE0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A4YVE0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U5JSS4

Plasmid

#163183

Purpose

Inducible expression of UniProt identifier A0A0U5JSS4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U5JSS4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0F0C8R9

Plasmid

#163192

Purpose

Inducible expression of UniProt identifier A0A0F0C8R9

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0F0C8R9

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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D3NRV6

Plasmid

#163195

Purpose

Inducible expression of UniProt identifier D3NRV6

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

D3NRV6

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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