We narrowed to 20,218 results for: ACE;

1. mADAM10 E385A in pQCXIP cFLAG

   Plasmid

   #19137

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E385A

   Promoter

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

2. MTK0_003

   Plasmid

   #123925

   Purpose

   Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cas9-sgRNA destination
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MTK0_051

   Plasmid

   #123948

   Purpose

   Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hAAVS1 landing pad destination vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tsPurple chromoprotein promoter-less

   Plasmid

   #118007

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAB07 (CK2beta)

   Plasmid

   #27091

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. Nuc-mTurq2-Apollo-NADP+

   Plasmid

   #193302

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   SV40 NLS and mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PLX306 iCre tetO NICD rtTA

   Plasmid

   #158049

   Purpose

   lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   mouse Notch Intra Cellular Domain
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCRE-iRFP670

   Plasmid

   #82696

   Purpose

   Expresses CRE and iRFP670 under two separate PGK promoters.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Insert

   CRE, iRFP670
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCK004 ubi:mCerulean_SV40 (#302)

   Plasmid

   #195978

   Purpose

   Expression construct

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubi:mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92228

    Purpose

    Expression of zmADH using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. TJP1_PDZ_2

    Plasmid

    #103910

    Purpose

    Protein expression and purification of ZO1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

    Plasmid

    #124865

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Expression

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-MfPylRS (human-opti)

    Plasmid

    #164081

    Purpose

    To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MfPylRS(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

    Plasmid

    #160740

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mADAM10 deltaMP in pQCXIP cFLAG

    Plasmid

    #19138

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 56-457

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

23. TJP1_PDZ_1

    Plasmid

    #103909

    Purpose

    Protein expression and purification of ZO1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MTK0_057

    Plasmid

    #123950

    Purpose

    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI LP on hAAVS1 (Hygro::mRuby)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MTK0_047

    Plasmid

    #123977

    Purpose

    Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hCLYBL CAS9 Destination - HygroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. T7-p73DD L371P-pcDNA3

    Plasmid

    #23049

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 327-…

    Promoter

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBeast-pAhpC-RBS- sfGFP

    Plasmid

    #190051

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCXLE-hK + E2-Crimson

    Plasmid

    #74946

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only