Purpose
Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
Mutation
amino acids 1005-1013 replaced with two glycine
Available Since
March 6, 2020
Availability
Academic Institutions and Nonprofits only