We narrowed to 12,311 results for: pcDNA

Showing: 4541 - 4560 of 12311 results

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pcDNA-emerin-EGFP-polyA

Plasmid

#59747

Purpose

Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A)

Plasmid

#14132

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1 151-241

Plasmid

#24390

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 151-241.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-NES-ZapCV2 (cpV143)

Plasmid

#112060

Purpose

Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

Depositor

Amy Palmer

Article

Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

Insert

NES-ZapCV2

Tags

Nuclear Export Signal (NES) derived from HIV-1

Expression

Mammalian

Mutation

ECFP gene is truncated 36 bp at 3' end. Venu…

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
hE-cadherin/α-catenin fusion-pcDNA3

Plasmid

#45771

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Human, Frog)

Expression

Mammalian

Mutation

Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp7 C186A-Flag

Plasmid

#11816

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Tags

Flag

Expression

Mammalian

Mutation

C186A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-EGFP-ELYS-polyA

Plasmid

#59746

Purpose

Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

EGFP-ELYS

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK7 D155A HA (P#812)

Plasmid

#14648

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Mutation

D155A: dominant negative

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
Yellow Cameleon-Nano140/pcDNA3

Plasmid

#51966

Purpose

mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano140

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3. Flag-CSL R192H

Plasmid

#167005

Purpose

Mammalian expression of CSL R192H

Depositor

Gian-Paolo Dotto

Article

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Insert

CSL (RBPJ Human)

Tags

FLAG

Expression

Mammalian

Mutation

R192H

Promoter

CMV

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-invitro(KaganD86)

Plasmid

#52012

Purpose

Used for in vitro expression of the human MAVS CDS

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1859A

Plasmid

#46239

Purpose

FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1859 changed to Alanine (S1859A)

Promoter

cmv

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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CHOP 6: mCHOP-WT-9E10-pcDNA1

Plasmid

#21913

Depositor

David Ron

Insert

CHOP10 (Ddit3 Mouse)

Tags

9E10 (Myc)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4(C744E)/2797

Plasmid

#11206

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

cysteine 744 mutated to glutamic acid

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag mTOR S2035T

Plasmid

#26604

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Mutation

S2035T

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
1552 pcDNA3 HA gamma adaptin 1

Plasmid

#10712

Depositor

William Sellers

Insert

Gamma adaptin 1 (AP1G1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S142A

Plasmid

#46771

Purpose

Expression of GAL4 fusion to CREB-S142A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S142A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 142 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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