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Showing: 4561 - 4580 of 19449 results
  1. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-Akt-STOPS-NES

    Plasmid
    #175008
    Purpose
    Cytosol-targeted Akt-STOPS.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pRK5-Ufm1 ∆C3

    Plasmid
    #133866
    Purpose
    Expression of human UFM1 mutant lacking the last three C-terminal residues
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. mCh-BICD2*-strep

    Plasmid
    #120168
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BICD cargo adaptor 2 (Bicd2 Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated BICD2: 15-595 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  9. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid
    #137722
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
    Depositor
    Insert
    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Truncated at amino acid 1328
    Promoter
    Tight TRE promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 4561 - 4580 of 19449 results