We narrowed to 24,455 results for: nes

1. MMLVgag-3NES-Cre

   Plasmid

   #207252

   Purpose

   Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   MMLVgag-3NES-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Use

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_CMV_MeltNLS/NES-40-mCherry

   Plasmid

   #234066

   Purpose

   Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltNLS/NES-40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. NES-mCherry-cPHx3

   Plasmid

   #116862

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem trimer)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. m6A-Tracer-NES

   Plasmid

   #159607

   Purpose

   Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   m6A-Tracer-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NES-mCherry-P4Mx1

   Plasmid

   #108143

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NES-Grx1-roGFP2

   Plasmid

   #236551

   Purpose

   Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   NES-Grx1-roGFP2 (NES Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEXP(V5-APEX2-NES)

   Plasmid

   #107596

   Purpose

   Expresses V5-APEX2-NES in mammalian cells

   Depositor

   Markus Hafner

   Article

   Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

   Insert

   APEX2-NES
   

   Use

   Frt site to insert into hek293 t-rex genome

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Dox inducible

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NES-EGFP-P4Mx1

   Plasmid

   #108121

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RANLS-DEVD-BNES

    Plasmid

    #50840

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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