We narrowed to 866 results for: seph

1. pRAU171

   Plasmid

   #86835

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA2

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-14-3-3dm (pEBG)

   Plasmid

   #1943

   Depositor

   Joseph Avruch

   Article

   Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

   Insert

   14-3-3 zeta (Ywhaz Mouse)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Dimerization-deficient mutant of 14-3-3zeta aa: E…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pTetO-Kir2.1-IRES-TLZ

   Plasmid

   #32642

   Depositor

   Joseph Gogos, C. Ron Yu, Richard Axel

   Article

   Yu et al Neuron. 2004 May 27;42(4):553-66.

   Insert

   tetO-Kir2.1-IRES-TLZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSW24

   Plasmid

   #86837

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA3

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSW65

   Plasmid

   #86838

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA3

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-MST1

   Plasmid

   #1965

   Depositor

   Joseph Avruch

   Article

   Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

   Insert

   MST1 (Stk4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MYC-MST1

   Plasmid

   #8847

   Depositor

   Joseph Avruch

   Article

   Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

   Insert

   MST1 (Stk4 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV-H2B-mRFP1

   Plasmid

   #47376

   Purpose

   This was used as a nuclear marker

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   H2B
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

   Plasmid

   #164263

   Purpose

   Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

   Depositor

   Joseph Peters

   Article

   Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DCX 3UTRm3hp

    Plasmid

    #28302

    Depositor

    Joseph Loturco

    Article

    Bai et al Nat Neurosci. 2003 Dec . 6(12):1277-83.

    Insert

    Doublecortin 3' Untranslated region mutated
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3 base pair mutation in DCX 3'UTR region

    Promoter

    Available since

    May 12, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-6xHis-TrxF

    Plasmid

    #207454

    Purpose

    E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

    Depositor

    Joseph Jez, Sarah Stainbrook

    Article

    Stainbrook et al bioRxiv 2022.08.24.505184

    Insert

    Thioredoxin F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    T7

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-Nix-S35A

    Plasmid

    #197562

    Purpose

    Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-35. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 35 changed to alanine

    Promoter

    CMV

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV10/3xflag-Clock

    Plasmid

    #47334

    Purpose

    full length clock in pCMV10 with 3x flag at the N-terminus

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    clock
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. plvx-tetO-GFP

    Plasmid

    #191560

    Purpose

    Inducible expression of GFP

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

    Insert

    gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJB06

    Plasmid

    #190480

    Purpose

    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Cas9
    XylR
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    xylR-driven promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-TID-HA3-HIS3MX6

    Plasmid

    #227981

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-TID-HA3-KanMX6

    Plasmid

    #227980

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WNK2 WT

    Plasmid

    #24569

    Depositor

    Joseph Costello

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

    Insert

    WNK2 (WNK2 Human)
    

    Use

    Tags

    His6 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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