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We narrowed to 17,736 results for: EMB

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Showing: 1101 - 1150 of 17736 results

  4. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid
    #154910
    Purpose
    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Synthetic, Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB
    Promoter
    CMV
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154910-plasmid-map-sequence-id-318440

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  5. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Expression
    Bacterial
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

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  11. pDyn1

    Plasmid
    #64067
    Purpose
    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    DYNC1H1 (DYNC1H1 Human)
    Use
    Cre/Lox
    Tags
    His-ZZ-TCS-SNAPf
    Expression
    Insect
    Mutation
    Codon optimized for Sf9
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    64067-plasmid-map-sequence-id-107133

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  13. pCS2+-Y276H PHYB-CAAX

    Plasmid
    #154911
    Purpose
    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154911-plasmid-map-sequence-id-308717

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  15. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  17. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  30. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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  31. A24M04 mIgM heavy chain-pFEEKW

    Plasmid
    #234809
    Purpose
    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 membrane IgM heavy chain (IGHM Human)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    EEK promoter
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234809-plasmid-map-sequence-id-466813

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  33. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid
    #58422
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids P29 to D408
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58422-plasmid-map-sequence-id-90253

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  41. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101851-plasmid-map-sequence-id-200251

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  42. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  43. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186355-plasmid-map-sequence-id-373423

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  45. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

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  46. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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  50. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58423-plasmid-map-sequence-id-123700

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Showing: 1101 - 1150 of 17736 results