Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 17,618 results for: URE

Clear keywords
Showing: 1101 - 1150 of 17618 results

  1. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

    Enlarge

  2. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

    Enlarge

  8. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid
    #47457
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47457-plasmid-map-sequence-id-69629

    Enlarge

  21. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47454
    Purpose
    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-SID4X
    Use
    AAV
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47454-plasmid-map-sequence-id-69624

    Enlarge

  23. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

    Enlarge

  28. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

    Plasmid
    #47453
    Purpose
    epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (N136,Grm2,C63)-cib1
    Use
    AAV; Tale
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47453-plasmid-map-sequence-id-72982

    Enlarge

  42. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50421
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50421-plasmid-map-sequence-id-79593

    Enlarge

Showing: 1101 - 1150 of 17618 results