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Showing: 441 - 460 of 11379 results

  1. pHEY1-donor

    Plasmid
    #112297
    Purpose
    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP
    Depositor
    Kevin White
    Insert
    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:79765311:G>A
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    112297-plasmid-map-sequence-id-225585

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  3. pGDP3 arr

    Plasmid
    #112890
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    arr
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112890-plasmid-map-sequence-id-219900

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  4. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  7. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

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  8. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Use
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112911-plasmid-map-sequence-id-220214

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  9. pSNAI2-donor

    Plasmid
    #112378
    Purpose
    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP
    Depositor
    Kevin White
    Insert
    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112378-plasmid-map-sequence-id-229744

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  12. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

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  15. pMM1157

    Plasmid
    #112534
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    luxCDABE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL(HrtO) and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112534-plasmid-map-sequence-id-216000

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  16. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

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  17. pRNATU6.1 HDAC3 shRNA2

    Plasmid
    #112271
    Purpose
    HDAC3 KD
    Depositor
    Simone Di Giovanni
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112271-plasmid-map-sequence-id-213643

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  20. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Kevin White
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112329-plasmid-map-sequence-id-228556

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Showing: 441 - 460 of 11379 results