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Showing: 441 - 460 of 12943 results

  2. pBCL6.1.0-gDNA

    Plasmid
    #112485
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6
    Depositor
    Kevin White
    Insert
    BCL6 (BCL6 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112485-plasmid-map-sequence-id-230527

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  5. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  7. CvcE in pGEX4t

    Plasmid
    #112977
    Purpose
    For protein expression and purification of Drosophila CvcE
    Depositor
    Terry Orr-Weaver
    Insert
    cycE (CycE Fly)
    Expression
    Bacterial
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112977-plasmid-map-sequence-id-217475

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  10. pOP5BT

    Plasmid
    #112609
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112609-plasmid-map-sequence-id-220239

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  14. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

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  16. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

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  17. mScarlet-I-LSS-SGFP2

    Plasmid
    #112945
    Purpose
    Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mScarlet-I Fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112945-plasmid-map-sequence-id-223563

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  18. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

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Showing: 441 - 460 of 12943 results