Addgene

We narrowed to 4,075 results for: PRS

Showing: 441 - 460 of 4075 results

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pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - pRSET

Plasmid

#193947

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2(3)

Plasmid

#202451

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2

Plasmid

#202449

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TMSNKRS

Plasmid

#198323

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_FGF21

Plasmid

#182406

Purpose

Bacterial expression of fgf21

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

fgf21 (FGF21 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_Uricase

Plasmid

#182400

Purpose

Bacterial expression of uricase

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

uricase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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ipUSEPR-sg-Hs-TMPRSS2-A5

Plasmid

#188689

Purpose

sgRNA

Depositor

Lewis Cantley

Insert

sgTMPRSS2 (TMPRSS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3-M129C

Plasmid

#132949

Purpose

Expresses tagless S3-M129C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

E.coli ribosomal protein S3 (rpsC E.coli)

Use

Tags

Expression

Bacterial

Mutation

M129C

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS11B

Plasmid

#172421

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 11B (TMPRSS11B Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

MET25

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-ChPylTMSK

Plasmid

#163915

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG

Plasmid

#158145

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BYG

Plasmid

#158144

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (wild-type)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA

Plasmid

#134673

Purpose

Expresses His6-TglA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB BS2mT4L1

Plasmid

#141203

Purpose

Chimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Planchestainer et al Catalysts 2018, 8(1), 40

Insert

BS2m-T4L1

Use

Tags

Expression

Bacterial

Mutation

E11Q

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_unstable

Plasmid

#127607

Purpose

high-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ECM33 intron (Mut)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-ECM33_intron

Plasmid

#127596

Purpose

low-copy URA-selectable plasmid, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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