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Plasmid#142338PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORB (RORB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-mTET1
Plasmid#83569PurposeExpresses TET1 with a mutated catalytic domain in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationcatalytic domain mutant H1672D, D1674APromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-WT(pL84)
Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorInsertGal4-TET1-878-911 wildtype (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_THRA_Hormone-recep
Plasmid#109810PurposeProtein expression and purification of THRA_Hormone-recepDepositorInsertTHRA_Hormone-recep (THRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
JA740
Plasmid#49939PurposeExpresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a controlDepositorInsertTet1CD GFP (TET1 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA524
Plasmid#49938PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a controlDepositorInsertTet1CD GFP (TET1 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKLF6 P148L (1009)
Plasmid#49497Purposeexpresses human KLF6 with P148L mutationDepositorInsertKFL6-P148L (KLF6 Human)
UseTagsExpressionMammalianMutationP148LPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-TET1-dCas9
Plasmid#235599PurposeExpresses Tet1CD-dCas9DepositorInsertTET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
UseCRISPR and LentiviralTags3xNLS and TET1 CDExpressionMammalianMutationPromoterEF1aAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_StrepII-Hook2_FTS_FHIP2A
Plasmid#222302PurposeCo-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vectorDepositorUseTagsStrepII-PscExpressionInsectMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S172A_M173A RAD23A
Plasmid#201574PurposeExpresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutations S172A and M173APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_V195A RAD23A
Plasmid#201575PurposeExpresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutation V195APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)
Plasmid#154875PurposeExpresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1DepositorInsertPfBAEBL (PF3D7_0506900 P. falciparum)
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationI1134V, P1135L, Y1136V, F1137V; insert codes for …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-I894A(pL84)
Plasmid#124404PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.DepositorInsertGal4-TET1-878-911(I894A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationI894APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-T898A(pL84)
Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationT898APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL900APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL897APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L900A(pL91)
Plasmid#124395PurposeE.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L900A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL900APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only