-
Plasmid#109546PurposeMultisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)DepositorInsertmKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRP6-m5-pCS2-VSVG
Plasmid#27283DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsVSVGExpressionMutationChanged S/T of all five PPP(S/T)P motifs to Alani…PromoterAvailable sinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF0337
Plasmid#143395PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMECOM (MECOM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0338
Plasmid#144261PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMECOM (MECOM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0339
Plasmid#143396PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMECOM (MECOM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC
Plasmid#32023DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaNdeltaC
Plasmid#32030DepositorInsertDCR1(107-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1delta2d
Plasmid#32028DepositorInsertDCR1(15-260)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1 DN T31N
Plasmid#10833DepositorInsertArf1 T31N (ARF1 Human)
UseTagsHAExpressionMammalianMutationT31N. Defective in GTP binding. Dominant negati…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
UseTags6xHis-TEVExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pAcrF1
Plasmid#89233PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF1
UseTags6xHis-TEV and FLAGExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dnm2a-DN
Plasmid#109572PurposeMultisite gateway vector for 3' tagging with dominant negative Dynamin2aDepositorInsertDynamin2a (dnm2a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV[FLEX]-CAG-EGFP-pri-miR-137
Plasmid#235159PurposeExpresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent mannerDepositorInsertEGFP/pri-miR-137 (Mir137 Mouse)
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE
Plasmid#235148PurposeExpresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporterDepositorInsertmCherry with a mutated miR-124 MRE
UseAAVTagsExpressionMutation7 nucleotides of the miR-124 WT MRE mutatedPromoterEFSAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
p3E-jph1a
Plasmid#109551PurposeMultisite gateway vector for 3' tagging with junctophilin 1aDepositorInsertjunctophilin 1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
-