We narrowed to 22,191 results for: his

1. pSRK51

   Plasmid

   #223722

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   Sreejith Raran-Kurussi

   Article

   Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

   Insert

   TEV protease
   

   Use

   Tags

   NT* (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   T7

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_Caspase-4_sheep_catalytic domain

   Plasmid

   #214331

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTcI39-PRM-wasabi

   Plasmid

   #195555

   Purpose

   Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIwt-PRM-Wasabi

   Plasmid

   #195553

   Purpose

   Always-on expression of mWasabi reporter with transcription activation by wildtype CI

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   CIwt
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_Caspase-11_rat_catalytic domain

   Plasmid

   #214329

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Rat)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_Pro-IL-18_lemur

   Plasmid

   #214332

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_Caspase-4_lemur_catalytic domain

   Plasmid

   #214328

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_Pro-IL-18_sheep

   Plasmid

   #214335

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_Pro-IL-18_rabbit

   Plasmid

   #214334

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_Pro-IL-18_dog

    Plasmid

    #214336

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_Caspase-4_rabbit_catalytic domain

    Plasmid

    #214330

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUAS-kkv-OH

    Plasmid

    #138956

    Purpose

    Used to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv with C terminal epitope tag (kkv Fly)
    

    Use

    Tags

    ollas and his-6 added to C terminus of protein co…

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SUMO_LPETG_MT3

    Plasmid

    #200304

    Purpose

    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJL1_CSL3

    Plasmid

    #200173

    Purpose

    Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

    Depositor

    Michael Jewett

    Article

    Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

    Insert

    CSL3
    

    Use

    Tags

    6x His tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM.F03R

    Plasmid

    #198664

    Purpose

    Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEM.F01R

    Plasmid

    #198660

    Purpose

    Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM.A01R

    Plasmid

    #198656

    Purpose

    Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    Adaptor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BPTI-21

    Plasmid

    #195058

    Purpose

    simplyfied amino acid sequence by alanine scanning

    Depositor

    Yutaka Kuroda, Peter Kim

    Article

    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

    Insert

    bovine pancreatic trypsin inhibitor
    

    Use

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    21 amino acids are Alanine out of 58 amino acids

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-15b(ETNPPL)

    Plasmid

    #192264

    Purpose

    Coding sequence of mouse ETNPPL is inserted in pET-15b vector

    Depositor

    Toshihide Yamashita

    Article

    Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

    Insert

    ethanolamine phosphate phospholyase (Etnppl Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTXTL-T7max-Luz

    Plasmid

    #188304

    Purpose

    Expression of Luz via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    luz
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

    Plasmid

    #183387

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    redesigned Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ANR261ENP, D315H, M318K, T352K

    Promoter

    T7

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TDRD3:ABC006-C02:C220502

    Plasmid

    #183982

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TDRD3-anti-TDRD3 FAB (TDRD3 Human)
    

    Use

    Tags

    anti-TDRD3 FAB HC: N-terminal STII leader peptid…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pMAL-ccDockerin

    Plasmid

    #72200

    Purpose

    MBP-ccDockerin expression plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

    Insert

    Clostridium Cellulolyticum Type I Dockerin
    

    Use

    Tags

    His-Tag and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-epELM-5

    Plasmid

    #179391

    Purpose

    Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

    Depositor

    Shiro Iuchi

    Article

    Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

    Insert

    ELMSAN1 (MIDEAS Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lmajor_gp63_10_0460_D463N

    Plasmid

    #171644

    Purpose

    Expression of L. major glcyoprotein-63 (D463N) with substrate binding site mutation from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

    Insert

    LmjF.10.0460 D463N
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    D463N

    Promoter

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lmajor_gp63_10_0460_P460G_D463N_S465A

    Plasmid

    #171645

    Purpose

    Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

    Insert

    LmjF.10.0460 P460G D463N S465A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    P460G; D463N; S465A

    Promoter

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NmMetNI-WT

    Plasmid

    #172114

    Purpose

    Expression of MetN and MetI membrane proteins

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Inserts

    MetN
    MetI
    

    Use

    Tags

    10x His Tag and Enterokinase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11a-Z-N-DiB2

    Plasmid

    #168473

    Purpose

    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

    Insert

    N-fragment of DiB2 + Leucine Zipper
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEL1305

    Plasmid

    #155143

    Purpose

    Expresses MlaFB, His tag on MlaF N-term

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (6xHis-TEV-MlaF)-MlaB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEL1306

    Plasmid

    #155144

    Purpose

    Expresses MlaFB, His tag on MlaF C-term

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (MlaF-6xHis)-MlaB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEL1307

    Plasmid

    #155145

    Purpose

    Expresses MlaFB, His tag on MlaB N-term

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEL1308

    Plasmid

    #155146

    Purpose

    Expresses MlaFB, His tag on MlaB C-term

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(MlaB-6xHis)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBEL1833

    Plasmid

    #155149

    Purpose

    Expresses MlaF(1-250)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-250)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJ414 - NERD-C

    Plasmid

    #136389

    Purpose

    Expresses NERD-C static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-C
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-F30L-V39I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. A102-GAP-BoCCS

    Plasmid

    #123287

    Purpose

    Expression of truncated Glo3 (1-375) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    1-375

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. A102-BoCCS-GRM

    Plasmid

    #123288

    Purpose

    Expression of truncated Glo3 (137-459) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    137-459

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. A102-Par-BoCCS-GRM

    Plasmid

    #123289

    Purpose

    Expression of truncated Glo3 (296-459) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    296-459

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. A102-BoCCS

    Plasmid

    #123290

    Purpose

    Expression of truncated Glo3 (137-375) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    137-375

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. A102-GRM

    Plasmid

    #123291

    Purpose

    Expression of truncated Glo3 (373-459) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    373-459

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmCellFree_KA1

    Plasmid

    #145369

    Purpose

    Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Type

    Empty backbone

    Use

    Tags

    His and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge