We narrowed to 32,111 results for: eng

1. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Expression

   Insect

   Promoter

   nanos

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-TagRFP-2A-BSD

   Plasmid

   #167930

   Purpose

   Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBE14

   Plasmid

   #174653

   Purpose

   In vivo Cre-lox circularization in E. coli

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   Cre
   

   Use

   Synthetic Biology

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-M2

   Plasmid

   #135583

   Purpose

   High eukaryotic protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Expression

   Insect

   Promoter

   polyhedrin of AcMNPV

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP_TOMM20_sfCherry2(11)

   Plasmid

   #83033

   Purpose

   Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   TOMM20_sfCherry+11
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pre_II_CS3_9072_v1

   Plasmid

   #126854

   Purpose

   9072 b scaffold from compatibility group 3, made by method II

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   II_CS3_9072_v1
   

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP_sfCherry2(11)_H2B

   Plasmid

   #82605

   Purpose

   Expresses sfCherry2(11) tagged H2B in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_H2B
   

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29b_1_anti-GFP-nanobody

   Plasmid

   #210394

   Purpose

   Express anti-GFP nanobody in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   1_anti-GFP-nanobody
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBudORF1-CH

    Plasmid

    #51290

    Purpose

    expresses the codon optimized ORF1 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1 ORF1 (L1RE1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA_AAVS1-T1

    Plasmid

    #41817

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. p177bp-LacOx50-Hyg

    Plasmid

    #210213

    Purpose

    Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORF2

    Plasmid

    #206026

    Purpose

    An expression vector for mouse LINE1 ORF2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF2 derived from mouse LINE1
    

    Expression

    Mammalian

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Expression

    Bacterial

    Promoter

    SP6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP_sfCherry2(11)_zyxin

    Plasmid

    #83034

    Purpose

    Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_zyxin
    

    Expression

    Mammalian

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Tags

    RFP

    Expression

    Worm

    Available Since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-PIK3CD

    Plasmid

    #174190

    Purpose

    Expresses PIK3CD in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3CD (PIK3CD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe

    Plasmid

    #243745

    Purpose

    PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTD

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

    Insert

    pvc13-Ad5_pvc10-HUHe
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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