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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMC-ME mCherry-P2A-NTRv2-pA

Plasmid

#200532

Purpose

mini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-P2A-NTRv2-pA

Plasmid

#241195

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ133B2.0

Plasmid

#99892

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLG327

Plasmid

#128797

Purpose

Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLiLucB

Plasmid

#31596

Depositor

Michele Calos

Article

Woodard et al PLoS One. 2010 . 5(6):e11367.

Insert

alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDrop

Plasmid

#184872

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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placZα-tdMCP

Plasmid

#191132

Purpose

Expression of tdMCP from a lacZα inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

dMCP_1

Use

Synthetic Biology

Promoter

lac-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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IO001: pMVP (R4-R3) eGFP

Plasmid

#121730

Purpose

pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGR

Plasmid

#46002

Purpose

Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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IG703: pMVP (L1-R5) TRE promoter

Plasmid

#121697

Purpose

pMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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