We narrowed to 32,111 results for: eng

1. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-PIK3CG

   Plasmid

   #174189

   Purpose

   Expresses PIK3CG in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

   Insert

   PIK3CG (PIK3CG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-PIK3R1

   Plasmid

   #174191

   Purpose

   Expresses PIK3R1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pgrac

   Available Since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA_GFP-T1

   Plasmid

   #41819

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBudORF2-CH

    Plasmid

    #51289

    Purpose

    expresses the codon optimized ORF2 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 ORF2 (L1RE1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH62-EBD

    Plasmid

    #49455

    Purpose

    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase

    Depositor

    Marc Gartenberg

    Article

    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

    Insert

    Cre-EBD fusion
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    Gal1 promoter

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK_Sox10_enh_84_Citrine

    Plasmid

    #130584

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox10_enh_84
    

    Use

    Enhancer reporter

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-DIO-GRAB_Ado1.0

    Plasmid

    #153291

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT09

    Plasmid

    #223381

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORF1

    Plasmid

    #206025

    Purpose

    An expression vector for mouse LINE1 ORF1

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1 derived from mouse LINE1
    

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p177bp-LacOx162-Hyg

    Plasmid

    #210215

    Purpose

    Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    162xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAct-Cas9

    Plasmid

    #62209

    Purpose

    Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Expression

    Insect

    Promoter

    act5C

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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