We narrowed to 61,045 results for: promoter
-
Plasmid#193004PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D2)DepositorPublicationInsertHydra Zic4 promoter
ExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D2Available sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD_Mini35s(-60,UTR) (GB0050)
Plasmid#68176PurposeProvides the CaMV Mini35S Promoter as a level 0 GoldenBraid partDepositorInsertCaMV Mini35S promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-Crestin-pro (JDW 1320)
Plasmid#229830PurposeA gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expressionDepositorInsertCrestin promoter, c-fos minimal promoter, and b-globin intron
UseGateway CloningAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti sfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164478PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsmCherry and superfolder GFPExpressionMammalianPromoterCMVAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#126217PurposeLabeling of inhibitory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxExpressionMammalianPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ALKBH5-dCas9
Plasmid#134783PurposeExpression fusion protein ALKBH5-dCas9 in Mammalian cellsDepositorInsertALKBH5 and inactive dCas9
ExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CXCL12-sfGFP
Plasmid#98961PurposeMammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12DepositorInsertCXCL12 (CXCL12 Human)
TagsSuperfolder GFPExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64
Plasmid#85424PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-7_synNotch
UseLentiviralPromoterPGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GRB2-GFP
Plasmid#86873PurposeTo express GRB2 with a GFP tag at C terminus.DepositorAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-Vps34
Plasmid#86749PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-US-ECasE
Plasmid#83961PurposePiggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.DepositorInsertERT2-spCas9-ERT2
ExpressionMammalianMutationplease see depositor comments belowPromoterU6 (gRNA), EF1a (ERT2-Cas9-ERT2)Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by