We narrowed to 25,161 results for: promoter

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 beta-catenin Y654E

Plasmid

#16073

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

Y654E, *(see below)

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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NK73

Plasmid

#176609

Purpose

To test bead engulfment of macrophages.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

SNAP-CD86-FCERG (CD86 Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I delta isoform

Plasmid

#22188

Depositor

Ananda Roy

Article

Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR_hEMSY

Plasmid

#125156

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

EMSY (EMSY Human)

Use

Gateway entry vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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USP19 sgRNA2

Plasmid

#78586

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA2 (USP19 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway entry vector

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crScaffold_SV40-BFP

Plasmid

#224860

Purpose

crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP3

Plasmid

#209965

Purpose

Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P25)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP7

Plasmid

#209969

Purpose

Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_fabZ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP8

Plasmid

#209970

Purpose

Contains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_gadB)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP9

Plasmid

#209971

Purpose

Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_ldhD)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP1

Plasmid

#209963

Purpose

Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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