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tdminiSOG-Actin-7

Plasmid

#57803

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-miniSOG-Vimentin-7

Plasmid

#55099

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

NM_003380.3 (VIM Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Zyxin-6

Plasmid

#55101

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Keratin-17

Plasmid

#55091

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-5x3-hMeCP2-delN256-G273X

Plasmid

#48090

Purpose

Bacterial expression of GST tagged AT-Hook2-G273X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-5x3-hMeCP2-delN256-R270X

Plasmid

#48089

Purpose

Bacterial expression of GST tagged AT-Hook2-R270X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-hMeCP2-G273X-R111G

Plasmid

#48087

Purpose

Mammalian expression of Gal4 tagged MeCP2-G273X-R111G

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

Gal4

Expression

Mammalian

Mutation

R111G mutant MeCP2, expresses AT-hook G273X

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-G1237A

Plasmid

#24733

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

G replaced with A at 1237 position resulting in G…

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C1240T

Plasmid

#24735

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

C replaced with T at position 1240 resulting in H…

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-G1238A

Plasmid

#24738

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

G replaced with A at position 1238 resulting in G…

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-LC3-RFP-LC3ΔG

Plasmid

#168997

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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GLI1_pLENTI-CAG-IRES-GFP

Plasmid

#176992

Purpose

Mammalian lentiviral expression vector encoding GLI1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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BACE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176942

Purpose

Mammalian expression vector encoding BACE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

BACE2 (BACE2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-BRCA1

Plasmid

#115491

Purpose

EGFP-BRCA1 expression vector. Parton clone BXY

Depositor

Rob Parton

Article

McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

Insert

BRCA1 (BRCA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S1009R (See depositor comments)

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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