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pcsII-MECL1-flag

Plasmid

#86774

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

immunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

unknown

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD6

Plasmid

#195844

Purpose

Expresses human FZD6 with an N-terminal HiBiT; contains M345L

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD6 (FZD6 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

M345L

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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p3HAT1Luc1

Plasmid

#236275

Purpose

Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

angiotensin II type 1 receptor (AGTR1 Human)

Tags

3xHA and Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-MYO1D

Plasmid

#125125

Purpose

Expresses human FLAG-MYO1D

Depositor

Bruce Beutler

Article

McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

Insert

MYO1D (MYO1D Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pWzl_CRKL-Y207F+W275L

Plasmid

#196022

Purpose

Expresses CRKL Y207F + W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBW_0065

Plasmid

#102817

Purpose

pVec8 containing Sr22 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Native

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0059

Plasmid

#102819

Purpose

pAGM4723 containing Sr35 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr35 (NEWENTRY Synthetic)

Use

Synthetic Biology

Promoter

Native

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Article

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-nAChr-Alpha6-mCherry

Plasmid

#50480

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Tags

mCherry fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only
AAV-Syn-TRPV1-P2A-DsRed

Plasmid

#200830

Purpose

Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-DsRed (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK1-beta

Plasmid

#197121

Purpose

Expression of MYC-tagged PSK1-beta in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

Insert

TAOK2 variant beta (aa1-1049) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Wild Type variant beta

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_EF1a-NAE1_IRES_Puro

Plasmid

#235659

Purpose

human NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

NAE1 (NAE1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBW_0141

Plasmid

#102820

Purpose

pVec8 containing Sr45 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Sr33

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_GFP

Plasmid

#78172

Purpose

Doxycyline regulated expression of GFP in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb2-flag

Plasmid

#86766

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 2 (PSMB2 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA-UGI-T2A-mTagBFP2

Plasmid

#221224

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
HA-PRAS40 WT

Plasmid

#86758

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pVC-DNAJB6b

Plasmid

#175065

Purpose

expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

DNAJB6b (DNAJB6 Human)

Tags

V5 and mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(R306C)

Plasmid

#110188

Purpose

Expression of mouse MeCP2 (R306C) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arg 306 Cys

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_13A_3D

Plasmid

#106279

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein and NLS

Expression

Mammalian

Mutation

Deleted amino acids 401-636, T24A, S209A, H212R, …

Promoter

EF1a/RPBSA

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-Y207F-V5

Plasmid

#196016

Purpose

Expresses CRKL Y207F, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF11

Plasmid

#44341

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF11 (TAF11 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook3

Plasmid

#198525

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook3 (HOOK3 Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD9

Plasmid

#195847

Purpose

Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD9 (FZD9 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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