We narrowed to 10,009 results for: ADA

1. eSpCas9-EF-Gsk3b(new)

   Plasmid

   #122341

   Purpose

   Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Gsk3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159809

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P-2_dCrk

   Plasmid

   #131146

   Purpose

   Expresses GST::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_LHX2

   Plasmid

   #167776

   Purpose

   Expresses LHX2 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   LHX2 (LHX2 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-bJun-C-mSOG-IRES-mCherry

   Plasmid

   #136600

   Purpose

   Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   bJun-C-mSOG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79619

   Purpose

   Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   3xFlag-PmCDA1

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pspCas9-POLI-ST2-com vector

   Plasmid

   #176234

   Purpose

   Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAVA-LGF2-Gal11p

   Plasmid

   #127481

   Purpose

   Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

   Depositor

   Joel Malek

   Article

   Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

   Insert

   LGF2-Gal11p (GAL11 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-ER-EmEGFP

    Plasmid

    #169000

    Purpose

    Express ER-EmEGFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
    

    Use

    Unspecified

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEX- HcRed -hLC3ΔG

    Plasmid

    #24992

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available Since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pST_CAHS3_HYPDU (pBS0333)

    Plasmid

    #185179

    Purpose

    For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS3_HYPDU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST_APOE_HUMAN_E114I (pBS0839)

    Plasmid

    #185274

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E114I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E114I

    Promoter

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-DVC1-Strep-HA

    Plasmid

    #113481

    Purpose

    Expression of human DVC1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    DVC1 (SPRTN Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

17. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV11K

    Plasmid

    #63184

    Purpose

    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-RIPK1 K115R

    Plasmid

    #78835

    Purpose

    To overexpress RIPK1 K115R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K115R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GST-hFIP200(1363-1370Mut)-MBP

    Plasmid

    #190038

    Purpose

    Expression of FIP200 mutant

    Depositor

    James Hurley

    Article

    Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    GST and MBP

    Expression

    Mammalian

    Mutation

    1363 RDKDLIES 1370 to GSSGGSSG

    Promoter

    CMV

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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