Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 13,996 results for: eng

Clear keywords
Showing: 4621 - 4640 of 13996 results

  4. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

    Enlarge

  6. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid
    #129394
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV and Cre/Lox
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129394-plasmid-map-sequence-id-259728

    Enlarge

  7. pUC19-E6MBW6

    Plasmid
    #103102
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103102-plasmid-map-sequence-id-209054

    Enlarge

  9. pCMV dCas9-MC (eGFP)

    Plasmid
    #89931
    Purpose
    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    dCas9-MC
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deactivated Cas9, fragment of M.SssI (residues 27…
    Promoter
    CMV
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89931-plasmid-map-sequence-id-177995

    Enlarge

  12. dTomato_LD 0_CS G_tTA-BFP

    Plasmid
    #58871
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58871-plasmid-map-sequence-id-94232

    Enlarge

  19. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

    Enlarge

Showing: 4621 - 4640 of 13996 results