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UAS-mNAMPT

Plasmid

#187887

Purpose

Gal4/UAS expression of mammalian NAMPT

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

mNAMPT

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

Plasmid

#182377

Purpose

Dual expression construct encoding 3xFLAG and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mCherry

Expression

Mammalian

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLG006 (Ec67 retron)

Plasmid

#157884

Purpose

Expresses the Ec67 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec67 retron

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_336

Plasmid

#179095

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

BE3.9 (Cas9-NG)

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

COX4 (COX4 Budding Yeast)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-FAK WT

Plasmid

#186148

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRDA_479

Plasmid

#179099

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (SpG)

Use

CRISPR and Lentiviral

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

Plasmid

#200630

Purpose

Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

iCasp9

Use

Lentiviral

Promoter

pTRE3G

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27

Plasmid

#163617

Purpose

mouse SNX27 cDNA N-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLCS110

Plasmid

#209331

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-SENP3

Plasmid

#34554

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

SENP3 (SENP3 Human)

Tags

EGFP

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET21b Apaf1 1-591

Plasmid

#26642

Depositor

Guy Salvesen

Article

Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

Insert

Apoptotic protease activating factor 1 (APAF1 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acids 1-591

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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