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CMV_Npu-saBE3.9max C-terminal

Plasmid

#137180

Purpose

Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_C-terminal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-saBE3.9max N-terminal

Plasmid

#137179

Purpose

Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_N-terminal

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGMKOSiE

Plasmid

#20865

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)

Expression

Mammalian

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEasiLV-MCS-coICP0fxe

Plasmid

#235546

Purpose

To express ICP0-FXE (ICP0 ∆106-149, doxycycline inducible)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Mutation

Deletion of the RING domain, ∆106-149.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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NSP6-FR-2Strep

Plasmid

#210317

Purpose

Expresses SARS-CoV-2 NSP6 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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YFVdelNS1-mVenus

Plasmid

#179950

Purpose

pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mVenus

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking Nonstructural protein 1 (NS1) but with mVenus

Use

Transneuronal tracing

Promoter

SP6

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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YFVdelNS1-mCherry

Plasmid

#179951

Purpose

pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

Use

Transneuronal tracing

Promoter

SP6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1NF

Plasmid

#175279

Purpose

AAV vector mediating inducible expression of the NS1 gene of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1 (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

tight TRE

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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GLG1-EGFP

Plasmid

#210372

Purpose

Expresses GLG1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

GLG1 (GLG1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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