-
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-luc2
Plasmid#173006Purposefirefly luciferase reporter vector with 10x clustered UAS binding sitesDepositorInsert10xUAS-luc2
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM534
Plasmid#112530PurposepZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanRDepositorInsertluxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO)Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
UseTagsExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…PromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromotertetO7 promoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA3mt
Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2 corrected
Plasmid#10822DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutation3 silent mutations in orfPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHC042 (∆phaCAB)
Plasmid#226208PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRESneo-FLAG/HA Ago1
Plasmid#10820DepositorInsertArgonaute 1 (AGO1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL3-EF1a-i53
Plasmid#216243PurposeExpress i53 in mammalian cells as a HDR enhancerDepositorInserti53
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold-TF-hRubicon
Plasmid#64147PurposeFor protein expression in E. coliDepositorInsertRubicon (RUBCN Human)
UseTags6xHis-tag and Trigger Factor (TF)ExpressionBacterialMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only