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pAAV-FLEXfrt-SaCas9-U6-sgGria2

Plasmid

#124868

Purpose

Mutagenesis of Gria2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria2 (Gria2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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p202 Dync1li1 wt

Plasmid

#26450

Depositor

Elizabeth Fisher

Insert

cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Article

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO mouse ULK2 shRNA 93

Plasmid

#27635

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 shRNA 93

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Talin1 head-mCherry

Plasmid

#212005

Purpose

Expression of mouse talin1 head (1-490)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

Insert

Mouse talin1 head domain (1-490) (Tln1 Mouse)

Tags

mCherry fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Sufu_pLENTI-CAG-IRES-GFP

Plasmid

#176993

Purpose

Mammalian lentiviral expression vector encoding Sufu

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Sufu (Sufu Mouse)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mWnt1

Plasmid

#176486

Purpose

mouse Wnt1 cDNA in TetO backbone

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

mouse Wnt1 cDNA (Wnt1 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC flag-mRAP1 I312R

Plasmid

#73138

Purpose

Expression of Flag-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1 KO

Plasmid

#218182

Purpose

To KO Hevin in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-C-mCherryTag

Plasmid

#218183

Purpose

To tag Hevin with mCherry at its C-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

G324D

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2N620K

Plasmid

#22631

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 N620K (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Asparagine 620 to Lysine

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
GST-TAZ

Plasmid

#19024

Depositor

Michael Yaffe

Article

Park et al J Biol Chem. 2004 Apr 23. 279(17):17384-

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only