We narrowed to 9,400 results for: Coli

1. pC0.070

   Plasmid

   #119652

   Purpose

   Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   BBa_B0061
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_CtCHAD-H253A-R256A-R260A

   Plasmid

   #127788

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

   Plasmid

   #127789

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

   Plasmid

   #127792

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in Arg3…

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET27b-ecDHFR-N23PP

   Plasmid

   #110828

   Purpose

   Bacterial expression of E.coli DHFR N23PP mutant

   Depositor

   Stephen Benkovic

   Article

   Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23; a proline residue inserted be…

   Promoter

   T7

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b+R3(R112C/T180C)C

   Plasmid

   #108194

   Purpose

   Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b+3(R112C/T180C)C

   Plasmid

   #108193

   Purpose

   Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b+2(L171C)X

   Plasmid

   #108192

   Purpose

   Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA2(L171C)-PdX fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   L171C (PCNA2); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b+R2(L171C)X

   Plasmid

   #108191

   Purpose

   Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA2(L171C)-PdX fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   L171C (PCNA2); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b+C3(R112C/T180C)X

    Plasmid

    #108196

    Purpose

    Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    P450cam-PCNA3(R112C/T180C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-Tam1

    Plasmid

    #101639

    Purpose

    Encodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    Tam1
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pThom77.3

    Plasmid

    #101636

    Purpose

    Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    amFP486/K68M
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    K68M

    Promoter

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC3R112C/T180C

    Plasmid

    #85088

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3

    Promoter

    T7 promoter

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His10-PS-SNAPf-9R (XSB525)

    Plasmid

    #78513

    Purpose

    Construct for expressing His10 - SNAPf -9R in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    SNAPf
    

    Use

    Tags

    His10 followed by a prescission protease claevage…

    Expression

    Bacterial

    Mutation

    9R added to the C-terminal of SNAPf

    Promoter

    Available Since

    June 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSETb-rsGreen0.9

    Plasmid

    #78179

    Purpose

    Expresses-rsGreen0.9 in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen0.9
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSETb-rsGreen0.7b

    Plasmid

    #78178

    Purpose

    Expresses rsGreen0.7b in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen0.7b
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24a_CDP-CHase

    Plasmid

    #73161

    Purpose

    Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

    Depositor

    Maurice Bessman, Sandra Gabelli

    Article

    Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pJK287

    Plasmid

    #71120

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK175

    Plasmid

    #71132

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST14-ARL6

    Plasmid

    #67380

    Purpose

    Expression of native hArl6 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pDEST14-ARL8B

    Plasmid

    #67383

    Purpose

    Expression of native hArl8b in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pDEST14-ARL14

    Plasmid

    #67385

    Purpose

    Expression of native hArl14 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL14 (ARL14 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 172 C to T; aa L58F

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pDEST14-ARFRP1

    Plasmid

    #67387

    Purpose

    Expression of native hArfrp in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only