We narrowed to 162,717 results for: addgene

1. pX330-PITCh-PXN

   Plasmid

   #227315

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of PXN (PXN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Marathon-RT pentamutant for Split-PE (pEH107)

   Plasmid

   #190110

   Purpose

   Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, N26R, D74R, N116K, N197R

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. BRCA2 A12.2 gRNA

   Plasmid

   #90551

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A12.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW6.01

   Plasmid

   #62882

   Purpose

   Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   GFP::MEG-3
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   meg-3

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-At4CL-CmCHS-MsCHI

   Plasmid

   #73394

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   CmCHS
   MsCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-cGASΔN-HA

   Plasmid

   #130912

   Purpose

   Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGASΔN
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Flag-Rheb

   Plasmid

   #165031

   Purpose

   expression of Flag tagged Rheb protein in mammalian cells

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PU.1-IRES-mCherry

    Plasmid

    #80140

    Purpose

    retroviral expression of PU.1 and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    PU.1 (Spi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMVd1-NLS-GAL4-iLight2-VP16

    Plasmid

    #219550

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. VCP F12.4 gRNA

    Plasmid

    #90939

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F12.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABCG1_pCSdest

    Plasmid

    #53952

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    ABCG1 (ABCG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

17. CD40L-MIEG-hCD4 (hCD4-CD40L)

    Plasmid

    #40355

    Depositor

    Alexander Dent

    Article

    Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

    Insert

    CD40L (Cd40lg Mouse)
    

    Use

    Retroviral

    Tags

    IRES hCD4

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-hygro LAP2

    Plasmid

    #15740

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LAP2 (CEBPB Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

20. tet_pLKO.1_puro_shLuc

    Plasmid

    #136587

    Purpose

    Expresses an inducible short hairpin targeting firefly luciferase sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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