We narrowed to 11,748 results for: pcDNA

1. pcDNA3.1-2xFLAG-2xSTREP-CDK4

   Plasmid

   #172615

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111926

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNAI-GAL4-CREB-S133A

   Plasmid

   #46770

   Purpose

   Expression of GAL4 fusion to CREB-S133A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
   

   Use

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 133 to Alanine

   Promoter

   CMV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 5' cMyc- SMCI-S966A

   Plasmid

   #32371

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   S996A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-FRB-LplA(W37V)

   Plasmid

   #35608

   Depositor

   Alice Ting

   Article

   Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

   Insert

   FRB-LplA(W37V)
   

   Use

   Tags

   FLAG (internal fusion), HA, and His Tag

   Expression

   Mammalian

   Mutation

   LplA has Trp37 to Val mutation

   Promoter

   CMV

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO GFP DNAJA4

   Plasmid

   #19494

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJA4 (DNAJA4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP Cav2.2 HA D665A pcDNA3

   Plasmid

   #206113

   Purpose

   expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional

   Depositor

   Annette Dolphin

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D665A

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. cacna2d1 R241A D491A HA pcDNA3

   Plasmid

   #206109

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A D491A

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc-p110α-CAAX

   Plasmid

   #39490

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

   Insert

   p110 (PIK3CA Bovine)
   

   Use

   Tags

   CAAX and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNAI-GAL4-CREB-S142A

    Plasmid

    #46771

    Purpose

    Expression of GAL4 fusion to CREB-S142A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 142 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-YOD1 C160S

    Plasmid

    #49785

    Purpose

    Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    C160S

    Promoter

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

12. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Use

    Tags

    GST and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.2/V5-DEST NET1A V5 WT

    Plasmid

    #69817

    Purpose

    Expresses NET1A-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    NET1A (NET1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2/V5 hsa-mir-214

    Plasmid

    #26313

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-214 (MIR214 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4-His-Max-A-PQBP1

    Plasmid

    #25802

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Apr 21. ():.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid

    #207153

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAQ (GNAQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position F(124)E

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

    Plasmid

    #207160

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNG2 (GNG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-terminally tagged with LSS-mKATE2

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Synthetic, Human)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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