We narrowed to 6,126 results for: tTA

Showing: 4681 - 4700 of 6126 results

Results per page:

Sort results by:

TFAP4_BHLH_5-2

Plasmid

#185056

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-HypaCas9-CfANLN_sgRNA

Plasmid

#183880

Purpose

pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB_ROSA26_SpCas9_gRNAs

Plasmid

#174703

Purpose

Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

Depositor

Beverly L. Davidson

Article

Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

Insert

SpCas9 dual gRNAs

Use

AAV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
IDA3::NeonGreen N-term (WS14)

Plasmid

#126875

Purpose

NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

NeonGreen

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-hsg(G:H)

Plasmid

#138259

Purpose

Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mCherry targeting sgRNAs G and H

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gh47

Plasmid

#106718

Purpose

expression of gRNA targeting ST6GALNAC6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GALNAC6 (ST6GALNAC6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-D4 miR30-SUV39h2 ts2

Plasmid

#115884

Purpose

SUV39h2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h2 shRNA (SUV39H2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHRB.1.0-gDNA

Plasmid

#112429

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB

Depositor

Kevin White

Insert

THRB (THRB Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge