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pSCT-GAL93-SFPQ

Plasmid

#46320

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

SFPQ (SFPQ Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_3

Plasmid

#78200

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,I76G,A197C)

Expression

Bacterial

Mutation

A17C,I76G,A197C (please see depositor comment bel…

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-FIH1

Plasmid

#21399

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT-GEM(1)

Plasmid

#62893

Purpose

Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pT-GEM(2)

Plasmid

#62894

Purpose

Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGreenII 0000 (No LUC)

Plasmid

#44465

Depositor

Roger Hellens

Type

Empty backbone

Use

Plant transformation

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.Flag.HERC2-HECT-WT.6xHis

Plasmid

#39227

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

T7

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

6xHis and YPet

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGAL4-DBD-MCS

Plasmid

#145245

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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