We narrowed to 9,825 results for: 158

1. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

   Plasmid

   #125148

   Purpose

   Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   AcrIIA4-P2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

   Plasmid

   #176743

   Purpose

   Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Cx43-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-NLS-gs10-cTRb413

   Plasmid

   #188261

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cTRb413 (TXNRD2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.175

   Plasmid

   #184996

   Purpose

   Express -Eco1 HEK3 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK3 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCHKO

   Plasmid

   #155048

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR358.4

    Plasmid

    #125114

    Purpose

    Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-liaR(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19.TRIM28.FKBP.knock-in.mRuby2

    Plasmid

    #185008

    Purpose

    For knocking in FKBP F36V P2A mRuby2

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mRuby2
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSECC-sgTom

    Plasmid

    #138661

    Purpose

    Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBG-PELP1

    Plasmid

    #65209

    Purpose

    Full length mammalian expression vector for hPELP1

    Depositor

    Ratna Vadlamudi

    Article

    Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

    Insert

    PELP1 (PELP1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSCL.177

    Plasmid

    #184998

    Purpose

    Express -Eco1 EMX1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    EMX1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgMLST8_1

    Plasmid

    #124905

    Purpose

    Targeting human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR357.10

    Plasmid

    #125106

    Purpose

    Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1.5 BV

    Plasmid

    #16048

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter region (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1585 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

21. LentiCRISPRv2puro-Tbxas1

    Plasmid

    #170305

    Purpose

    A knock-out vector for the mouse Tbxas1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxas1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. trLATmEos3.2

    Plasmid

    #203745

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAT (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sfGFP-SasG(8G)-mCherry

    Plasmid

    #206950

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (8G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (8G)-mCherry
    

    Use

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-evoA1-seBE4max-IRES

    Plasmid

    #174699

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    evoA1-seBE4max-IRES
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    UGI-E11K

    Promoter

    CMV

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CABE-T1.17

    Plasmid

    #193260

    Purpose

    Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.17
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKH162_FLAG-APEX2-YIPF3 LIR mut

    Plasmid

    #214981

    Purpose

    Flag-APEX2 tagged LIR mutant YIPF3

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-APEX2-YIPF3_mut (YIPF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    LIR mutant

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTBL437 AAVS1 EQR sgRNA

    Plasmid

    #126447

    Purpose

    To cut the human AAVS1 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human AAVS1 safe-harbor locus with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXPR_003 sgMYC guide 2

    Plasmid

    #193597

    Purpose

    cMYC knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgMYC guide 2 (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge