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We narrowed to 9,625 results for: bli

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Showing: 7051 - 7080 of 9625 results

  1. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid
    #80077
    Purpose
    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection
    Depositor
    Phillip Abbosh
    Inserts
    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    Use
    Retroviral
    Tags
    5' FLAG
    Expression
    Mammalian
    Mutation
    Y641F, R342R, N393N
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    80077-plasmid-map-sequence-id-151639

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  2. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  4. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid
    #105639
    Purpose
    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    GFP YTA
    Expression
    Mammalian
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105639-plasmid-map-sequence-id-203817

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  5. RHPMP133 (mOrange2-beta Actin)

    Plasmid
    #88837
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Tags
    mOrange2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88837-plasmid-map-sequence-id-177529

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  6. Hs.KRAS4b G12S

    Plasmid
    #83144
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G12S
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83144-plasmid-map-sequence-id-158107

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  7. Lamin A (G465D)-mRFP

    Plasmid
    #124271
    Purpose
    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (G465D) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (G465D)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124271-plasmid-map-sequence-id-241962

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  8. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

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  9. ALDH18A1

    Plasmid
    #38947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH18A1 (ALDH18A1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. KSR2-HaloTag Fusion Vector

    Plasmid
    #236793
    Purpose
    Express KSR2-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KSR2 (KSR2 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236793-plasmid-map-sequence-id-471810

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  12. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  13. pFRT-TODestRFP_HuD

    Plasmid
    #65766
    Purpose
    expresses RFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Expression
    Mammalian
    Mutation
    V23M mutation in RFP
    Promoter
    CMV, 2x Tet
    Available Since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. MS2_SC2_S_Delta

    Plasmid
    #175950
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Delta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175950-plasmid-map-sequence-id-345521

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  16. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  17. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid
    #78336
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Promoter
    TRE2
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. Hs.KRAS4b G12R

    Plasmid
    #83143
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G12R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83143-plasmid-map-sequence-id-158106

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  19. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  20. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

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  21. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

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  22. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Expression
    Insect
    Promoter
    CMV and Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  23. ERAP1

    Plasmid
    #39174
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ERAP1 (ERAP1 Human)
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Promoter
    polyhedrin
    Available Since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NW3 (KMT2A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  26. pET28a-UBC9

    Plasmid
    #228949
    Purpose
    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228949-plasmid-map-sequence-id-472392

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  27. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  28. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid
    #230056
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230056-plasmid-map-sequence-id-457171

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  29. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

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Showing: 7051 - 7080 of 9625 results