We narrowed to 12,952 results for: sequence

1. SBP-mCherry-Ecadherin_puromycin

   Plasmid

   #65293

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]

   Plasmid

   #133329

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All FYW to S in CTD of full-length TDP43

   Promoter

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

   Plasmid

   #133328

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All F to S in CTD of full-length TDP43

   Promoter

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Or1a-Gal4

   Plasmid

   #63019

   Purpose

   To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

   Insert

   Drosophila melanogaster Or1a promoter
   

   Use

   Tags

   Drosophila ADH (aa's 1-18) fused to Yeast Ga…

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pENV8(GAAA)-GFPuv

   Plasmid

   #70128

   Purpose

   Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

   Depositor

   Robert Batey

   Article

   Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

   Insert

   kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaSpeR4 mito roGFP2-Orp1

   Plasmid

   #65001

   Purpose

   Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGAGmEos2-N1

   Plasmid

   #46372

   Depositor

   Suliana Manley

   Article

   Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

   Insert

   Gag
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Rev independentHXB2 Gag sequence

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH760-CEN-mCherry_v3

   Plasmid

   #40598

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mCherry ORF partially codon optimised for yeast e…

   Promoter

   GPD (TDH3)

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK_Citrine_Nanotag_23_neg_Control

   Plasmid

   #130562

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   negative oligo sequence
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TAL-L1-Tf1-Ctrl

    Plasmid

    #103036

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-Tf1
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-AILR-LplA-ER

    Plasmid

    #42574

    Depositor

    Alice Ting

    Article

    Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

    Insert

    LplA(AILR) with ER-retention sequence KDEL
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Compared to wild-type LplA: tryptophan 37 to alan…

    Promoter

    CAG

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pT2-cryR;tmem88aCE1-P1Egfp

    Plasmid

    #90148

    Purpose

    contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    tmem88aE1P1Egfp (tmem88a Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-L1PA8WTmneo

    Plasmid

    #69609

    Purpose

    Expresses a neomycin tagged wildtype L1PA8

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA8
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cyto-RA

    Plasmid

    #209860

    Purpose

    Dimerization dependent fluorescent protein RA fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    NES sequence NLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Use

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GLURP-bio-His

    Plasmid

    #50802

    Purpose

    Expresses enzymatically monobiotinylated full length GLURP

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    GLURP
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS1413 GFP-TDP43 VLIM-F

    Plasmid

    #118802

    Purpose

    To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Prohibitin-bio-His

    Plasmid

    #50800

    Purpose

    Expresses enzymatically monobiotinylated full length Prohibitin

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    Prohibitin
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBH31(gfcD)

    Plasmid

    #176199

    Purpose

    Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBH31(gfcD-10xHis)

    Plasmid

    #176200

    Purpose

    Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAPM D4 BsmBI Myd88 stuffer

    Plasmid

    #128505

    Purpose

    pAPM knockdown vector with Myd88 stuffer sequence

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    Puromycin Resistance
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Tags

    Expression

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Promoter

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSG5-ERRbeta-delta10

    Plasmid

    #52187

    Purpose

    Expresses human ERRbeta-delta10 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

25. V1-MESA-45F-M-dCas9

    Plasmid

    #84504

    Purpose

    MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTK_Citrine_Nanotag_5_neg_Control

    Plasmid

    #130558

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHBS1411 GFP-TDP43 FYW-S

    Plasmid

    #118800

    Purpose

    To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    All FYW to S in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

    Plasmid

    #133333

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All W to S in CTD of full-length TDP43

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCHA1.1-Rap2a-V5

    Plasmid

    #149509

    Purpose

    Stable expression of human Rap2a.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    RAP2 (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHBS1415 GFP-TDP43 VLIM-S

    Plasmid

    #133323

    Purpose

    To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ALL VLIM in the TDP43 IDRs mutated to S

    Promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge