We narrowed to 165,307 results for: addgene

1. pAGK111

   Plasmid

   #228126

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   synapsin I

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

   Plasmid

   #158988

   Purpose

   Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-KRAB
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-puro LEF1

   Plasmid

   #27023

   Depositor

   Joan Massague

   Article

   Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-miSFIT-2x

   Plasmid

   #124691

   Purpose

   Tuning expression of EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol1-UAS:GtACR2-tdTomato

   Plasmid

   #124236

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   GtACR2-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Promoter

   14xUAS-E1b

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tornado-RNA sensor for MALAT-1

    Plasmid

    #185406

    Purpose

    circular RNA-scaffolded sensing modules for MALAT-1

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    circular RNA-scaffolded sensing modules
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    U6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-flex-MCS--4x6T

    Plasmid

    #196420

    Purpose

    Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRL_U2AF1_S34F_mCherry

    Plasmid

    #84019

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34F

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eBFP2 (eBFP2)_CD

    Plasmid

    #66034

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - BFP
    

    Use

    Synthetic Biology

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p300 KAT LIC 1M

    Plasmid

    #233587

    Purpose

    Bacterial expression of p300 KAT enzyme

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300 KAT (EP300 Human)
    

    Tags

    6xHis MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. CSTB_pCSdest

    Plasmid

    #53910

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CSTB (CSTB Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAE1 G8.3 gRNA

    Plasmid

    #90777

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NAE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NAE1 (Guide Designation G8.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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