We narrowed to 162,717 results for: addgene

1. pSLIK 3xFLAG-BCAS2 zeo

   Plasmid

   #192319

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-BCAS2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BCAS2 (BCAS2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2-CAG-fGFP

   Plasmid

   #108714

   Purpose

   Transposon donor plasmid for long-term expression of membrane targetted GFP

   Depositor

   Madeline Lancaster

   Article

   Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

   Insert

   EGFP
   

   Use

   Tags

   6xHis and farnesylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDYH5

   Plasmid

   #52032

   Purpose

   Expression of GFP-tagged DAF-16 in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   DAF-16 (daf-16 Nematode)
   

   Use

   Tags

   GFP and HIS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

4. gh19

   Plasmid

   #106703

   Purpose

   expression of gRNA targeting GALNT19

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GALNT19
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-pMecp2-SaCas9-pU6-sgRNA

   Plasmid

   #159173

   Purpose

   Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p-mCherry2-sgRNA (empty)

   Plasmid

   #198330

   Purpose

   Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   U6-sgRNA(F+E) empty
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Hmga2 3'UTR m7 luciferase (Luc-m7)

   Plasmid

   #14788

   Depositor

   David Bartel

   Article

   Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

   Insert

   Luc-m7 (Hmga2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   m7 mutant (see article and author's map for …

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pICSL12009

   Plasmid

   #68257

   Purpose

   Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter and 5UTR Ubiquitin (Zea mays)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gh171

   Plasmid

   #106832

   Purpose

   expression of gRNA targeting UGCG

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   UGCG (UGCG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-CAG-dDIO-MCS--4x6T

    Plasmid

    #196422

    Purpose

    Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330-PITCh-MYO1C

    Plasmid

    #227305

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DKC1 F1.3 gRNA

    Plasmid

    #90652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KIF20A D5.2 gRNA

    Plasmid

    #90718

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF20A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF20A (Guide Designation D5.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DKC1 F2.3 gRNA

    Plasmid

    #90653

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN_TT 3xFLAG-KPNB1

    Plasmid

    #192301

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-hSyn-Gβγ-BERKY1

    Plasmid

    #158429

    Purpose

    BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK3ct
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. AAV-CAG-flex-lck-smV5

    Plasmid

    #196423

    Purpose

    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV and Cre/Lox

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge