We narrowed to 13,726 results for: Cas9

1. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

   Plasmid

   #199489

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

   Plasmid

   #199497

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. vHB66

   Plasmid

   #193456

   Purpose

   Vector for expression of second copy genes in pandoravirus/mollivirus

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Type

   Empty backbone

   Use

   Pandoravirus/mollivirus expression

   Tags

   Expression

   Mutation

   Promoter

   pneo650

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS621

   Plasmid

   #188893

   Purpose

   ttTi5605 MosSci targeting vector with floxed Cbr-unc-119 marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Type

   Empty backbone

   Use

   Mossci targeting vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBoL1P3GA

   Plasmid

   #112909

   Purpose

   Express a cloned sgRNA from AtU626 promoter

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

   Insert

   AtU626-LACZ-sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   At U626

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBoL1P4GA

   Plasmid

   #112910

   Purpose

   Express a cloned sgRNA from AtU626 promoter

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

   Insert

   AtU626 PROMOTER-Laz-sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   AtU626

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL12022

   Plasmid

   #117510

   Purpose

   AtAG promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pAG:AATG:BsaI
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtAG

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWT007h

   Plasmid

   #107892

   Purpose

   W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-SD8-Cas9, PLac-sgRNA(con.)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP574-1

   Plasmid

   #99477

   Purpose

   3XFlag::Topaz::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::Topaz::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAP996-1

    Plasmid

    #99499

    Purpose

    3XFlag::meGFP::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::meGFP::linker::TEV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP835-1

    Plasmid

    #99495

    Purpose

    TEV::meGFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::meGFP::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMLS292

    Plasmid

    #73725

    Purpose

    SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-mCherry + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ286

    Plasmid

    #52227

    Purpose

    Expression of sgRNA precursor targeted against ade6L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CR102

    Plasmid

    #234043

    Purpose

    expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

    Depositor

    Alexander Marson

    Article

    Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

    Insert

    TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPtGE35

    Plasmid

    #107999

    Purpose

    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Tags

    Cas9

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AID-C12*-BE4max

    Plasmid

    #216729

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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