We narrowed to 18,470 results for: Met

1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-8

   Plasmid

   #129060

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA8 (mouseTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

   Plasmid

   #129040

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA12 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

   Plasmid

   #129042

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA2 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

   Plasmid

   #129033

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA5 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

   Plasmid

   #129034

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA6 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

   Plasmid

   #129035

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA7 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

   Plasmid

   #129037

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA9 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

   Plasmid

   #129038

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA10 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

   Plasmid

   #129039

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA11 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid

    #129030

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA2 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid

    #129031

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA3 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid

    #129032

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA4 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEAHISMRSATAG

    Plasmid

    #97004

    Purpose

    Expressing MRSA TAG protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    DNA-3-methyladenine glycosylase I
    

    Tags

    N-ter TEV protease cleavable 6His

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9-DNMT3A-EGFP

    Plasmid

    #71666

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-MCAM-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156909

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    MCAM (MCAM Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3HA-ERHBD-Hoxb8

    Plasmid

    #212932

    Purpose

    Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

    Depositor

    Hans Haecker

    Article

    Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

    Insert

    3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Triple HA tag

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBoBi-hLAMP2-C-GC6s

    Plasmid

    #154151

    Purpose

    To detect the calcium ion releases close to the lysosomal surface

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    LAMP2 and GCamp6s (LAMP2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84481

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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