We narrowed to 18,890 results for: Tec

1. pJL-SaBE3

   Plasmid

   #85169

   Purpose

   Expresses SaBE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaBE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB508-cTP-mCherry

   Plasmid

   #213862

   Purpose

   Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

   Insert

   Plastid transit peptide fused mCherry-UnaG
   

   Expression

   Bacterial and Plant

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-APOBEC1-YTHmut

   Plasmid

   #200159

   Purpose

   For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHmut
   

   Tags

   6xHis, HA, and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT13-splitC

   Plasmid

   #122596

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. hSyn-IRES2-GFP

   Plasmid

   #234875

   Purpose

   Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSYn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscALPSpuro-Nipah V

   Plasmid

   #174228

   Purpose

   Expresses Nipah virus V

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   Nipah V
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTCON2-BYG

   Plasmid

   #158126

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   mTagBFP
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLO1811_arC9_noNLS_human

    Plasmid

    #74494

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    CRISPR

    Tags

    t2a mCherry (C-terminal on insert)

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM441

    Plasmid

    #61271

    Purpose

    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

    Plasmid

    #141048

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF1-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYC3.60-C1

    Plasmid

    #67899

    Purpose

    Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    YC3.60
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-Flp-DOG

    Plasmid

    #80084

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    Flp-DOG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH-dCas9-PBE

    Plasmid

    #98162

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Rice

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFYF1328 EGFP Site#3

    Plasmid

    #47513

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. CP1028-CBEmax

    Plasmid

    #119802

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1028) -UGI-UGI
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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