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V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pmScarlet_H2A_C1

Plasmid

#85051

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-Puro

Plasmid

#170069

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

Cas9 guide RNA scaffold with the F+E scaffold modification

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200406

Purpose

Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

MCP-ADAR2dd(E488Q)-NES

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

E488Q (hyperactive)

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Green Glifon4000

Plasmid

#126208

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon4000

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3nls

Plasmid

#155366

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX3_FOXO1

Plasmid

#205875

Purpose

Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX3_FOXO1 (PAX3, FOXO1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Use

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJL 89

Plasmid

#98558

Purpose

Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Enh 35S

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR

Plasmid

#112175

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFR

Plasmid

#65225

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term StrepII eGFP 3C

Plasmid

#160683

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

StrepII eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2-GFP

Plasmid

#202194

Purpose

knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP knock-in

Expression

Mutation

Promoter

lac

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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p156

Plasmid

#186179

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 156

Use

Synthetic Biology and Unspecified; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p136

Plasmid

#186177

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 136

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p166

Plasmid

#186180

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 166

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1237

Plasmid

#119262

Purpose

for cloning new sgRNAs

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183523

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

I146M/G174S

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG.CASP3-Myc

Plasmid

#200119

Purpose

Expresses C-terminal Myc-tagged caspase 3 in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

caspase 3 (CASP3 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

Plasmid

#172640

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-phSyn-flex-SypEGFP

Plasmid

#153203

Purpose

Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Lynette Lim

Insert

synaptophysin-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

rat synapsin

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pNTI820

Plasmid

#216629

Purpose

cpTurboID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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