159,557 results

1. Myosin-IIA-GFP

   Plasmid

   #38297

   Depositor

   Matthew Krummel

   Article

   Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

   Insert

   Myosin II A (Myh9 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1(+)-NEMOb

   Plasmid

   #189934

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag-HIF1B (#930)

   Plasmid

   #99916

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-fDIO-oG-WPRE-SV40pA

   Plasmid

   #74291

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   CAG

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Fon/ConN2cG

   Plasmid

   #234709

   Purpose

   pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

   Depositor

   David Ng

   Article

   Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

   Insert

   Fon Con N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-HisD-mNG-Ec-Kbp-D9N

   Plasmid

   #178011

   Purpose

   Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   mNG-Ec-Kbp-D9N
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-Cytochrome C

   Plasmid

   #41181

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C (CYCS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti-fullef1a-FAKSPARK2

    Plasmid

    #195265

    Purpose

    express wtFAKSPARK2 in a lenti vector

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    FAKSPARK2 sensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ef1a

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-mEGFP-SQSTM1-WT

    Plasmid

    #194582

    Purpose

    Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

    Plasmid

    #231602

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag_codon_optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130995

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE-Tn5-165

    Plasmid

    #118545

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAc5.1B-EGFP

    Plasmid

    #21181

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    enhanced green flourescent protein
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV PIG (Puro IRES GFP)

    Plasmid

    #18751

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3

    Plasmid

    #48743

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +1060)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

24. mCherry-2A-eGFP(W58X) pcDNA3.1(-)

    Plasmid

    #172229

    Purpose

    Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    mCherry-2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3E-FRT-KanR-FRT pA

    Plasmid

    #82601

    Purpose

    3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLp_3050sNuc

    Plasmid

    #122030

    Purpose

    Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

    Depositor

    Vincent Eijsink, Geir Mathiesen

    Article

    Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

    Insert

    signal peptide Lp_3050
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sppA

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTagRFP-C1-Fibrillarin

    Plasmid

    #70649

    Purpose

    Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS426GFP-2×PH(PLCδ)

    Plasmid

    #36092

    Depositor

    Scott Emr

    Article

    Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

    Insert

    2xPH(PLCdelta) fusion
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    contains aa11-140 of PLCδ (including the PH domai…

    Promoter

    PRC1

    Available since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLC-EGFP-RIP3

    Plasmid

    #75165

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIP3

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIP3 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge