We narrowed to 143,213 results for: ins

1. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1_hPMS2

   Plasmid

   #129425

   Purpose

   Expression human PMS2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human PMS2 (PMS2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB1_hMLH1

   Plasmid

   #129426

   Purpose

   Expression human MLH1 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MLH1 (MLH1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-N-I-N-CAAX

   Plasmid

   #41686

   Purpose

   Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   Dronpa145N-IntersectinDH-Dronpa145N-CAAX
   

   Use

   Tags

   CAAX box

   Expression

   Mammalian

   Mutation

   K145N on Dronpa

   Promoter

   CMV

   Available since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-PIMT

   Plasmid

   #34852

   Depositor

   Bruce Downie

   Article

   Dinkins et al Plant J. 2008 Jul;55(1):1-13.

   Insert

   PIMT (PCMT1 Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pFB1_hMSH2

   Plasmid

   #129423

   Purpose

   Expression human MSH2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MSH2 (MSH2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200406

   Purpose

   Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   MCP-ADAR2dd(E488Q)-NES
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   E488Q (hyperactive)

   Promoter

   CMV

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHM6-Q23

   Plasmid

   #40263

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. scFv-GCN4-sfGFP-TET1CD

   Plasmid

   #184439

   Purpose

   TET1 catalytic domain fused with sfGFP and scFv against GCN4

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   scFv-GCN4-sfGFP-TET1CD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS59

    Plasmid

    #215680

    Purpose

    Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pmTurquoise2-C1

    Plasmid

    #60560

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBLADE_ONLY_C

    Plasmid

    #168050

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    3xP3

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmTurquoise2-N1

    Plasmid

    #60561

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

    Plasmid

    #232800

    Purpose

    Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    OsTIR1 F74G EGFP-AID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_human CDK1

    Plasmid

    #118597

    Purpose

    One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    guide RNA against human CDK1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-RT005-HaloGPM

    Plasmid

    #163385

    Purpose

    2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PT7-deGFP-MGapt

    Plasmid

    #67741

    Purpose

    T7 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T7term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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